SERPINH1 Antikörper (AA 247-418)

Produktnummer ABIN3043331

Produktdetails anti-SERPINH1 Antikörper

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Bindungsspezifität: AA 247-418

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SERPINH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Serpin H1(SERPINH1) detection. Tested with WB, IHC-P in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Serpin H1(SERPINH1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Protein Name: Serpin H1

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Hsp47 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Andere Bezeichnung: SERPINH1 (SERPINH1 Produkte)

Synonyme: AsTP3 antikoerper, CBP1 antikoerper, CBP2 antikoerper, HSP47 antikoerper, OI10 antikoerper, PPROM antikoerper, RA-A47 antikoerper, SERPINH2 antikoerper, gp46 antikoerper, SERPINH1 antikoerper, DKFZp468B197 antikoerper, cb216 antikoerper, hsp antikoerper, hsp47 antikoerper, id:ibd5060 antikoerper, wu:fc56b02 antikoerper, BERF-1 antikoerper, Cbp1 antikoerper, Cbp2 antikoerper, Hsp47 antikoerper, J6 antikoerper, Serpinh2 antikoerper, colligin antikoerper, astp3 antikoerper, cbp1 antikoerper, cbp2 antikoerper, pig14 antikoerper, pprom antikoerper, ra-a47 antikoerper, serpinh2 antikoerper, serpin family H member 1 antikoerper, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b antikoerper, serpin H1 antikoerper, selenoprotein K antikoerper, serine (or cysteine) peptidase inhibitor, clade H, member 1 antikoerper, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog antikoerper, SERPINH1 antikoerper, serpinh1b antikoerper, LOC100009353 antikoerper, Selenok antikoerper, Serpinh1 antikoerper, serpinh1.L antikoerper

Substanzklasse: Viral Protein

Hintergrund: Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen, thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.

Synonyms: 47 kDa heat shock protein antibody|47 kDa heat shock protein precursor antibody|Arsenic transactivated protein 3 antibody|Arsenic-transactivated protein 3 antibody|AsTP 3 antibody|AsTP3 antibody|CBP 1 antibody|CBP 2 antibody|CBP1 antibody|CBP2 antibody|Cell proliferation-inducing gene 14 protein antibody|Collagen binding protein 1 antibody|Collagen binding protein 2 antibody|Collagen binding protein antibody|Collagen-binding protein 2 antibody|Collagen-binding protein antibody|Colligen antibody|Colligin 1 antibody|Colligin 2 antibody|Colligin antibody|colligin-1 antibody|colligin-2 antibody|gp46 antibody|Heat shock protein 47 antibody|Heat-shock protein 47 antibody|HGNC 1547 antibody|Hsp 47 antibody|J6 antibody|OI10 antibody|PIG 14 antibody|PIG14 antibody|PPROM antibody|Proliferation inducing gene 14 antibody|Proliferation inducing gene 14 protein antibody|RA A47 antibody|RA-A47 antibody|Rheumatoid arthritis antigen A 47 antibody|rheumatoid arthritis antigen A-47 antibody|Rheumatoid arthritis related antigen RA A47 antibody|Rheumatoid arthritis-related antigen RA-A47 antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody|Serine or cysteine proteinase inhibitor clade H member 1 antibody|Serine or cysteine proteinase inhibitor clade H member 2 antibody|SERPH_HUMAN antibody|Serpin H1 antibody|Serpin peptidase inhibitor clade H member 1 antibody|serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|Serpin peptidase inhibitor, clade H, member 1 antibody|SERPINH1 antibody|SERPINH2 antibody

Gen-ID: 871

UniProt: P50454