COL4A1 Antikörper (AA 1445-1669)

Produktnummer ABIN3042755

Produktdetails anti-COL4A1 Antikörper

Target: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Bindungsspezifität: AA 1445-1669

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB, IHC-P in Human.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB, IHC-P in Human.
Gene Name: collagen, type IV, alpha 1
Protein Name: Collagen alpha-1(IV) chain

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Collagen IV is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-COL4A1 Antikörper (ABIN3042755)

Guo, Lin, Shao, Rong, Zhang: "BMP‑7 suppresses excessive scar formation by activating the BMP‑7/Smad1/5/8 signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1957-1963, (2018) (PubMed).

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Chen, Kong, Liu, Gao, Zhang, Li, Liu: "In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 95-104, (2017) (PubMed).

et al.: "Erratum: Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway ..." in: International journal of nanomedicine, Vol. 12, pp. 1515, (2017) (PubMed).

Zhang, Wang, Cui, Zhang, Liu, Chu, Liu, Zhang, Chu: "Continuing treatment with Salvia miltiorrhiza injection attenuates myocardial fibrosis in chronic iron-overloaded mice." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124061, (2016) (PubMed).

Su, Wei, Wei, Liu, Li: "Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6407-15, (2016) (PubMed).

Yao, Liu, Li, Hao: "Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5828-36, (2016) (PubMed).

Shan, Zhou, Xia, Qian: "Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1611-1616, (2016) (PubMed).

Zhao, Liu, Shi, Xie, Cui, Li, Li, Ren: "Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 945-950, (2016) (PubMed).

Zhang, Teng, Liu, Zhang, Liu: "Gene expression profile analyze the molecular mechanism of CXCR7 regulating papillary thyroid carcinoma growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 16, (2015) (PubMed).

Luo, Deng, Lamsal, Xu, Xiang, Cheng: "AMP-activated protein kinase alleviates extracellular matrix accumulation in high glucose-induced renal fibroblasts through mTOR signaling pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 1, pp. 191-200, (2015) (PubMed).

Qiu, Zhang, Zhao, Zhang, Wu, Guo, Hu: "Enhancement of endothelial differentiation of adipose derived mesenchymal stem cells by a three-dimensional culture system of microwell." in: Biomaterials, Vol. 53, pp. 600-8, (2015) (PubMed).

Yan, Zhang, Kolkin, Wang, Xia, Fan: "Mechanisms of nerve capping technique in prevention of painful neuroma formation." in: PLoS ONE, Vol. 9, Issue 4, pp. e93973, (2015) (PubMed).

Xu, Zhang, Xu, Guo, Wang, Wu, Wang, Luo, Zhou: "Antiphotoaging effect of conditioned medium of dedifferentiated adipocytes on skin in vivo and in vitro: a mechanistic study." in: Stem cells and development, Vol. 24, Issue 9, pp. 1096-111, (2015) (PubMed).

Yang, Ni, Duan, Wang, Chen, Chen, Chaugai, Zeldin, Tang, Wang: "CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages." in: Prostaglandins & other lipid mediators, Vol. 116-117, pp. 64-75, (2015) (PubMed).

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Li, Kong, Ma, Gao, Ma: "Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar fibroblasts." in: International journal of clinical and experimental medicine, Vol. 8, Issue 6, pp. 8551-60, (2015) (PubMed).

Liu, Lu, Li, Wei, Li: "Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation." in: Acta biomaterialia, Vol. 11, pp. 114-25, (2014) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Wenbin, Guojun: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).

Details zu COL4A1

Target: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Andere Bezeichnung: COL4A1 (COL4A1 Produkte)

Synonyme: col4a1 antikoerper, wu:fc02g11 antikoerper, HANAC antikoerper, ICH antikoerper, POREN1 antikoerper, arresten antikoerper, Bru antikoerper, Col4a-1 antikoerper, Del(8)44H antikoerper, Raw antikoerper, Svc antikoerper, collagen, type IV, alpha 5 (Alport syndrome) antikoerper, collagen type IV alpha 1 chain antikoerper, collagen, type IV, alpha 1 antikoerper, col4a5 antikoerper, COL4A1 antikoerper, Col4a1 antikoerper

Hintergrund: COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Synonyms: Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|COL4A2 antibody|COL4A3 antibody|COL4A4 antibody|COL4A5 antibody|Collagen Alpha 1(IV) Chain antibody|Collagen Alpha 2(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen IV Alpha 2 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Of Basement Membrane Alpha 2 Chain antibody|Collagen Type IV Alpha 1 antibody|Collagen Type IV Alpha 2 antibody|Collagen Type IV Alpha 3 antibody|Collagen Type IV Alpha 4 antibody|Collagen Type IV Alpha 5 antibody|DKFZp686I14213 antibody|FLJ22259 antibody

Gen-ID: 1282

UniProt: P02462

Pathways: Skeletal Muscle Fiber Development, Growth Factor Binding