Microphthalmia-Associated Transcription Factor (MITF) Antikörper

Antigen: Microphthalmia-Associated Transcription Factor (MITF)

Synonyme: MI, WS2A, bHLHe32, Wh, bw, mi, vit, Bhlhe32, Vitiligo, MGC124309, MGC124310, MITF, CMI9, MITF-H, MITF-M, ws2a, mitfa, MITF-A, LOC100313655, mitfb

Klonalität: Monoklonal (D5)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN303608

Produktbeschreibung

Weitere Bezeichnung: Microphthalmia (MiTF)

Immunogen: BALB/C mice were injected with N-terminal fragment of human Mi protein.

Format: Ig-fraction

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: D5

Spezifität: This antibody reacts with a 52-56 kD protein known as Microphthalmia (Mi). This antibody reacts with both melanocytic and non-melanocytic isoforms of Mi. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Transkriptionsfaktoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Hemesath, Price, Takemoto et al.: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." in: Nature, Vol. 391, Issue 6664, pp. 298-301, 1998 (PubMed).

Weilbaecher, Hershey, Takemoto et al.: "Age-resolving osteopetrosis: a rat model implicating microphthalmia and the related transcription factor TFE3." in: The Journal of experimental medicine, Vol. 187, Issue 5, pp. 775-85, 1998 (PubMed).