TUBA1B Antikörper (Tubulin, alpha 1B) (Biotin)

Details for Product anti-TUBA1B Antibody No. ABIN303511, Anbieter: Anmelden zum Anzeigen
Antigen
  • K-ALPHA-1
  • k-alpha-1
  • tuba
  • tuba1
  • Tuba2
  • RGD1565476
  • TUBA3
  • TUBAT
  • similar to alpha tubulin
  • alpha tubulin
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • tubulin, alpha 8
  • RGD1560000
  • LOC100009327
  • TUBA1B
  • tuba1b
  • Tuba1b
  • TUBA8
Reaktivität
Alle Spezies
48
21
17
7
5
5
5
5
3
3
3
2
2
2
1
1
1
Wirt
Maus
32
22
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TUBA1B Antikörper ist konjugiert mit Biotin
2
2
1
1
1
1
1
Applikation
Western Blotting (WB)
53
29
27
18
17
12
9
7
7
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Klon TU-01
Isotyp IgG1
Spezifität The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Andere Bezeichnung alpha Tubulin / TUBA1B (TUBA1B Antibody Abstract)
Hintergrund The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain
Gen-ID 10376
NCBI Accession NP_006073
UniProt P68363
Pathways Microtubule Dynamics, M Phase
Applikationshinweise Western Blotting (1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Allgemeine Veröffentlichungen Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997 (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, 1997 (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

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