V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA) Antikörper

Antigen: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA)

Synonyme: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN303337

Produktbeschreibung

Produktmerkmale: Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3

Weitere Bezeichnung: RELA / NF-kB p65

Swiss-Prot: Q04206

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the humanprotein conjugated to Keyhole Limpet Hemocyanin (KLH).

Kreuzreaktivität: Human

Beschreibung: NFkB was originally identified as a factor that binds to the immunoglobulin kappa lightchain enhancer in B cells. It was subsequently found in non-B cells in an inactivecytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as aheterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1)subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel,and RelB subunits are responsible for transactivation. The p50 and p52 subunits possessDNA binding activity but limited ability to transactivate. p52 has been reported to formtranscriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physicalinactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclearlocalization and DNA binding. Low levels of p52 and p50 homodimers can also exist incells.

Spezifität: Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Immunohistochemistry on Paraffin Sections (1/500). Immunoprecipitation. Immunofluorescence. Western Blot. ELISA. Gel Supershift. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Lagerung: Store vial at -20°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar