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Keratin Acidic (AE1) Antikörper

AE1 Reaktivität: Human, Ratte IF, WB, IHC (p) Wirt: Maus Monoclonal ACCK1-1 unconjugated
Produktnummer ABIN3026303
  • Target Alle Keratin Acidic (AE1) Antikörper anzeigen
    Keratin Acidic (AE1)
    Reaktivität
    • 32
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Human, Ratte
    Wirt
    • 18
    • 11
    • 3
    Maus
    Klonalität
    • 32
    Monoklonal
    Konjugat
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Unkonjugiert
    Applikation
    • 24
    • 24
    • 24
    • 20
    • 12
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.
    Klon
    ACCK1-1
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product AE1 Primärantikörper
  • Applikationshinweise
    Titering of the Acidic Cytokeratin antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    3. This antibody will detect the following cytokeratins (CK): CK10: 56  kDa, CK14: 50  kDa, CK15: 50  kDa, CK16: 48  kDa, CK19: 40  kDa.\. Western blot: 1-2 μg/mL (3),Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Keratin Acidic (AE1)
    Andere Bezeichnung
    Acidic Cytokeratin (AE1 Produkte)
    Hintergrund
    There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]
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