TGFA Antikörper (C-Term)

Produktnummer ABIN3026161

Produktdetails anti-TGFA Antikörper

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.

Klon: MF9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha (TGFA Produkte)

Synonyme: TFGA antikoerper, LOC100009482 antikoerper, TGF alpha antikoerper, TGFA antikoerper, wa-1 antikoerper, wa1 antikoerper, RATTGFAA antikoerper, TGFAA antikoerper, NV15606 antikoerper, si:ch211-10e8.7 antikoerper, transforming growth factor alpha antikoerper, transforming growth factor alpha L homeolog antikoerper, transforming growth factor-alpha antikoerper, transforming growth factor, alpha antikoerper, TGFA antikoerper, TGF-A antikoerper, Tgfa antikoerper, tgfa.L antikoerper, LOC100121300 antikoerper, LOC100009150 antikoerper, tgfa antikoerper

Hintergrund: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Gen-ID: 7039

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway