GABA B Receptor GB2 Subunit Antikörper

Antigen: GABA B Receptor GB2 Subunit

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN302104

Produktbeschreibung

Immunogen: Synthetic peptide (coupled with THG) derived from the last 23 aa of mouse GABA Breceptor 2. 100% homology with human GB2.

Format: Purified

Beschreibung: GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric acid(GABA), and has important functions in brain by inhibition of adenylyl cyclase andmodulation of G-protein-gated Ca 2+ and K + channels. GABA B receptor is comprised of two subunits, GB1 and GB2 with N-terminal extracellular and C-terminal intracellular domains.The GB1 subunit plays a critical role in ligand binding, whereas the GB2 subunit containsthe determinants required for G-protein signaling. Multiple allosteric interactions between thetwo subunits are required for correct functioning of the receptor. There are two N-terminalsplice variants of GB1 subunit, termed GB1a and GB1b, their expression in the centralnervous system changes during the ontogenesis and differs between various regions of thebrain.

Spezifität: The polyclonal antibody recognizes C-terminus of gamma-aminobutyric acid (GABA) Breceptor 2 (recognized epitope: the last 23 aa). GB2 apparent MW ~120 kDa.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 0.6 µg/ml

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from rabbit serum by protein-G affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blotting analysis of GABA B receptor GB2 subunit in rat liver (1), GB2-transfected HEK292 cells (2) and rat brain (3) with polyclonal anti-GB2 antibody.