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GFP Antikörper

GFP Reaktivität: Aequorea victoria WB, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN302066
  • Target Alle GFP Antikörper anzeigen
    GFP (Green Fluorescent Protein (GFP))
    Reaktivität
    • 179
    • 19
    • 15
    • 15
    • 12
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Aequorea victoria
    Wirt
    • 75
    • 74
    • 35
    • 11
    • 9
    • 6
    • 5
    • 1
    Kaninchen
    Klonalität
    • 122
    • 89
    Polyklonal
    Konjugat
    • 105
    • 14
    • 12
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GFP Antikörper ist unkonjugiert
    Applikation
    • 172
    • 87
    • 56
    • 42
    • 33
    • 32
    • 32
    • 21
    • 16
    • 14
    • 14
    • 14
    • 12
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Spezifität
    The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
    Kreuzreaktivität (Details)
    Recognizes fusion proteins in all species
    Aufreinigung
    Purified from rabbit serum by affinity chromatography
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    EGFP, a native full-length protein
    Top Product
    Discover our top product GFP Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Porrero, Rubio-Garrido, Avendauno, Clascua: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, (2010) (PubMed).

    Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

  • Target
    GFP (Green Fluorescent Protein (GFP))
    Andere Bezeichnung
    GFP (GFP Produkte)
    Synonyme
    green fluorescent protein antikoerper, gfp antikoerper
    Substanzklasse
    Viral Protein
    Hintergrund
    Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
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