NET1 Antikörper (AA 611-624)

Produktnummer ABIN299305

Dieser Ziege Polyklonal Antikörper detektiert spezifisch NET1 in ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NET1 Antikörper (AA 611-624) (ABIN299305)

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NET1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NET1 Antikörper

Bindungsspezifität: AA 611-624

Spezifität: Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Orangutan, Gibbon, Monkey (93%) Marmoset, Mouse, Hamster (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKALSGGKRKETLV from the C-terminus of human NET1 (NP_001040625.1, NP_005854.2). Percent identity by BLAST analysis: Human, Gorilla (100%), Orangutan, Gibbon, Monkey (93%), Marmoset, Mouse, Hamster (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Andere Bezeichnung: NET1

Hintergrund: Name/Gene ID: NET1

Synonyms: NET1, ARHGEF8, NET1A, Proto-oncogene p65 Net1

Gen-ID: 10276

NCBI Accession: NP_001040625, NP_005854

UniProt: Q7Z628

Pathways: Neurotrophin Signalübertragung