NLRP3 Antikörper (AA 82-93)

Produktnummer ABIN297366

Der Ziege Polyklonal anti-NLRP3 Antikörper (ABIN297366) detektiert spezifisch NLRP3 in ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für NLRP3 Antikörper (AA 82-93) (ABIN297366)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NLRP3 Antikörper

Bindungsspezifität: AA 82-93

Spezifität: Human NLRP3. This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bat, Panda, Pig (92%) Dog, Bovine, Rabbit (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bat, Panda, Pig (92%), Dog, Bovine, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NALP3 / NLRP3

Hintergrund: Name/Gene ID: NLRP3

Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS

Gen-ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001230062, NP_001120933, NP_001120934

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome