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FHL1 Antikörper

FHL1 Reaktivität: Human, Maus, Ratte WB, IF, IC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN7306298
  • Target Alle FHL1 Antikörper anzeigen
    FHL1 (Four and A Half LIM Domains 1 (FHL1))
    Reaktivität
    • 44
    • 15
    • 13
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 34
    • 6
    • 5
    Kaninchen
    Klonalität
    • 38
    • 7
    Polyklonal
    Konjugat
    • 34
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FHL1 Antikörper ist unkonjugiert
    Applikation
    • 34
    • 17
    • 12
    • 7
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)
    Spezifität
    Recognizes endogenous levels of FHL-1 protein.
    Produktmerkmale
    Rabbit polyclonal antibody to FHL-1
    Aufreinigung
    The antibody was purified by immunogen affinity chromatography.
    Immunogen
    Recombinant full length protein of human FHL-1
    Top Product
    Discover our top product FHL1 Primärantikörper
  • Applikationshinweise
    WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #101371 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo
    No.
    #101371
    Datum
    19.07.2017
    Antigen
    FHL-1
    Chargennummer
    AL1F04A
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    MRL/lpr lupus mice (14 weeks of age)
    Negativkontrolle
    FH knockout MRL/lpr lupus mice
    Bewertung
    Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2966633
    Sekundärantikörper
    goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
    Full Protocol
    • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
    • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
    • Sections were cut at 8µm using the cryostat.
    • Dry sections for 30min at RT.
    • Hydrate sections in PBS 2x for 15min at RT.
    • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
    • Wash sections 2x with PBS for 15min at RT.
    • Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
    • Wash sections 2x for 15min with PBS.
    • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
    • Wash sections 2x for 15min with PBS.
    • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
    • Wash sections 2x for 15min with PBS.
    • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
    Anmerkungen
  • Format
    Liquid
    Buffer
    Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    FHL1 (Four and A Half LIM Domains 1 (FHL1))
    Andere Bezeichnung
    FHL-1 (FHL1 Produkte)
    Synonyme
    fhl1 antikoerper, MGC89165 antikoerper, FHL1 antikoerper, DKFZp468O1927 antikoerper, fhl antikoerper, fhla antikoerper, zgc:92025 antikoerper, FHL-1 antikoerper, FHL1A antikoerper, FHL1B antikoerper, FLH1A antikoerper, KYOT antikoerper, SLIM antikoerper, SLIM-1 antikoerper, SLIM1 antikoerper, SLIMMER antikoerper, XMPMA antikoerper, KyoT antikoerper, Fhl1 antikoerper, four and a half LIM domains 1 antikoerper, four and a half LIM domains 1a antikoerper, Four and a half LIM domains protein 1 antikoerper, four and a half LIM domains 1 S homeolog antikoerper, fhl1 antikoerper, FHL1 antikoerper, fhl1a antikoerper, fhl1.S antikoerper, Fhl1 antikoerper
    Hintergrund
    SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1
    Gen-ID
    2273, 14199, 25177
    UniProt
    Q13642, P97447, Q9WUH4
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