SMAD6 Antikörper (AA 85-99)

Produktnummer ABIN295868

Der Kaninchen Polyklonal anti-SMAD6 Antikörper wird verwendet zum Nachweis von SMAD6 in Proben von Human und Schwein. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für SMAD6 Antikörper (AA 85-99) (ABIN295868)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reaktivität: Human, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMAD6 Antikörper

Bindungsspezifität: AA 85-99

Spezifität: A 52 kD band is observed.

Homologie: Percent identity by BLAST analysis: Human, Pig (100%) Dog, Bovine, Opossum (93%) Gibbon, Marmoset, Rat (87%) Elephant (80%).

Aufreinigung: Protein G purified

Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%), Dog, Bovine, Opossum (93%), Gibbon, Marmoset, Rat (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Andere Bezeichnung: SMAD6

Hintergrund: Name/Gene ID: SMAD6

Synonyms: SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6

Gen-ID: 4091

UniProt: O43541

Pathways: Chromatin Binding