SORBS1 Antikörper (AA 449-462)

Produktnummer ABIN290372

Der Ziege Polyklonal anti-SORBS1 Antikörper wird verwendet zum Nachweis von SORBS1 in Proben von Human, Hund, Pferd und Affe. Er wurde validiert für ELISA, IHC und IHC (p).

Kurzübersicht für SORBS1 Antikörper (AA 449-462) (ABIN290372)

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Reaktivität: Human, Hund, Pferd, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SORBS1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SORBS1 Antikörper

Bindungsspezifität: AA 449-462

Spezifität: Human SORBS1. This antibody is expected to recognise all 7 reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Elephant, Horse (100%) Rat, Opossum (93%) Mouse (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-SPKSEDDDSDLYSP from an internal region of human SORBS1 (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Elephant, Horse (100%), Rat, Opossum (93%), Mouse (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SORBS1

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Andere Bezeichnung: SORBS1 / Ponsin

Hintergrund: Name/Gene ID: SORBS1

Synonyms: SORBS1, C-Cbl associated protein, C-Cbl-associated protein, CAP, FLAF2, KIAA0894, KIAA1296, R85FL, SH3 domain protein 5, SH3-domain protein 5 (ponsin), SH3P12, SORB1, Fas-ligand associated factor 2, Ponsin, SH3D5

Gen-ID: 10580

NCBI Accession: NP_006425, NP_056200, NP_001030126, NP_001030127, NP_001030128, NP_079267, NP_001030129

UniProt: Q9BX66

Pathways: Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process