Free Fatty Acid Receptor 1 (FFAR1) Antikörper

Antigen: Free Fatty Acid Receptor 1 (FFAR1)

Synonyme: Gpr40, FFAR1, GPR40

Klonalität: Monoklonal (G16)

Wirt: Maus

Reaktivität: Maus, Human

Applikation: Durchflusszytometrie (FACS), Immunzytochemie (ICC)

Produktnummer: ABIN290047

Produktbeschreibung

Weitere Bezeichnung: GPR40

Immunogen: Partial peptide of Mouse GPR40

Format: Affinity-purified

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: G16

Beschreibung: G protein coupled receptors (GPCRs) are a protein family of transmembrane receptors, and involved in a widevariety of physiological processes. GPR40 is also a member of GPCRs and functions as a receptor for long-chainfree fatty acids (FFA), which is expressed in beta cells in the islets of pancreas and is involved in the regulationof glucose and lipid metabolisms. Long chain FFAs amplify glucose-stimulated insulin secretion from pancreaticbeta cells by activating GPR40.GPR40 knockout mice fed a high-fat diet are protected against obesity-induced hyperinsulinemia and hepaticsteatosis. Transgenic mice overexpressed GPR40 in pancreatic beta cells had impaired beta cell function anddeveloped diabetes.GPR40 is an important mediator of both the acute, stimulatory effects and chronic,deleterious effects of FFAs on beta cells.Moreover, GPR40 may be implicated in the control of breast cancer cell growth by fatty acids and provide a linkbetween fat and cancer.This antibody is specific to GPR40 and will be useful to research for lipid metabolism, obesity and diabetes.

Anwendungen

Applikationshinweise: Working dilution For Immunocytochemistry 1.0 ug/ml. For Fluorescence activated cell sorting 0.5 ug/ml

Konzentration: 0.25 mg/ml

Reinigung: The splenic lymphocytes from GANP mouse, immunized with the first extracellulardomain peptides of mouse GPR40 conjugated with KLH, were fused to myeloma P3U1cells. The screening of the hybridoma cells was performed on ELISA. The cell line with positive reaction was grown on non-serum medium, from which the antibody waspurified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunocytochemical staining of HEK293 cellsoverexpressing human GPR40. Tsujimoto G., Genomic Drug Discovery Science,Kyoto University Graduate school of PharmaceuticalSciences, Kyoto, Japan