Ca2+ / Calmodulin-Dependent Protein Kinase II delta1-delta4 Antikörper

Antigen: Ca2+ / Calmodulin-Dependent Protein Kinase II delta1-delta4

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN289976

Produktbeschreibung

Immunogen: Partial peptide of Rat Ca2+ / Calmodulin-Dependent Protein Kinase II delta1-delta4

Format: Affinity-purified

Beschreibung: Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) may play key roles in various Ca2+-induced cellular functions. Interestingly, many kinds of isoforms have been identified in various tissues orcells. Recently, it has been revealed that some isoforms are located in specific regions in the cells, so it isspeculated that these isoforms have physiologically particular functions in each region.There are four different isoforms such as alpha,beta,gamma and delta. This antibody reacts with delta 1 to delta4 splice variants. Immunochemical studies indicate that delta2 is expressed in various tissues or cells such asinsulinoma cells and that delta3 is abundant in the nucleus in cerebellar granule cells. These results suggest that delta3 is involved in Ca2+ -dependent gene expression.This antibody has been proved to be useful for the immunoblotting and immunohistochemistry.

Anwendungen

Applikationshinweise: Working dilution Immunohistochemistry: 15ug/ml ,for immunoblotting: 10-20ug/ml

Konzentration: 1 mg/ml

Reinigung: Antigen Affinity

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoblotting Sample: (1) MIN6 (control) (2) MIN6 (after overexpression of delta 2). Preparation of antibodies and instruction: Hideyuki Yamamoto Department of Pharmacology and Neuropsychiatry,Faculty of Medicine, Kumammoto University, Japan