PEDF Antikörper

Antigen: PEDF

Klonalität: Monoklonal (14K)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN289972

Produktbeschreibung

Immunogen: Partial peptide of Human PEDF-KLH

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 14K

Beschreibung: Pigment Epithelium-Derived Factor ( PEDF ), a protein previously shown to have neurotrophic activity,is indispensable for neuronal development, differentiation and existence adjacent to retina.The amount of inhibitory PEDF produced by retinal cells was positively correlated with oxygenconcentrations,suggesting that its loss play a permissive role in ischemia-driven retinal neovascularization.So that, PEDF is likely to contribute to regulate of blood vessels growth in eye by creating a permissiveenvironment for angiogenesis when oxygen is limiting and an inhibitory environment oxygenconcentrations are normal or high ( Science Vol 285: 245-248,1999).PEDF may be a therapeutic target pathologic ocular neovascularization as well as for retinoblastomas.This antibody could detect PEDF with western-blotting method in medium of retinal pigment epitheliumcell. This antibody is useful in research for angionenesis and neuronal system.

Anwendungen

Konzentration: 0.25 mg/ml

Reinigung: The cell line was grown on serum free medium, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 0.1% BSA as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Sample: PEDF in the medium of retinal pigment epithelium. Primary antibody: (a) Control mouse IgG (b) Anti PEDF monoclonal antibody. 2nd antibody: (a,b) HRP labeled anti mouse IgG