Nitrotyrosine Antikörper

Antigen: Nitrotyrosine

Klonalität: Monoklonal (2H1)

Wirt: Maus

Produktnummer: ABIN289971

Produktbeschreibung

Immunogen: Nitrotyrosine-HSA

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 2H1

Beschreibung: To elucidate the function of Nitric Oxide (NO) related signal transduction, we developed a new monoclonal antibody to Nitrotyosin (Clone No.2H1). There are two pathways, which isengaged in the signal transduction regarding vascular relaxation on endothelial cell. One isthrough activated guanlyate cyclase that is cGMP dependent and another is cGMPindependent pathway which requires reactive NO derived substance such as,peroxinitrate (ONOO), N2O3,N2O4(NO2).Recently, various functions of reactive NO derived substance has been identified forexample regulating protein phosphorylation and inducing apoptosis. So, it is believed thatreactive NO derived substance is involved in signal transduction among cells.Immunohystochemical study revealed that nitotyrosin residue is produced in some patientsuch as athrosclerosis, Alzheimer

Anwendungen

Konzentration: 0.25 mg/ml

Reinigung: The spleen cells from BALB/c mouse, immunized with nitrotyrosin-HSA, were fused tomyeloma P3U1 cells. The screening of the hybridoma cells was performed on ELISA.The cell line was grown on non-serum medium, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 0.1% BSA as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar