CEL Antikörper

Antigen: CEL

Klonalität: Monoklonal (KNH-30)

Wirt: Maus

Reaktivität: Alle Spezies

Applikation: Immunhistochemie (IHC), ELISA

Produktnummer: ABIN289959

Produktbeschreibung

Immunogen: CEL-BSA

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: KNH-30

Beschreibung: Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base andAmadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recentimmunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteinsin several human tissues: human lens (nondiabetic and noncataractous), renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves ofdiabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrilsin patients with hemodialysis-related amyloidosis, senile plaques of patients with Alzheimer

Anwendungen

Applikationshinweise: Working dilution for immunohistochemistry: 5-10ug/ml, for ELISA: 0.1-1.0ug/ml

Konzentration: 0.25 mg/ml

Reinigung: The splenic lymphocytes from BALB/c mouse, immunized with CEL-BSA were fused tomyeloma P3U1 cells. The cell line (KNH-30) with positive reaction was grown in ascitic fluid ofBALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ling, Nagai, Sakashita et al.: "Immunohistochemical distribution and quantitative biochemical detection of advanced glycation end products in fetal to adult rats and in rats with streptozotocin-induced diabetes." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 81, Issue 6, pp. 845-61, 2001 (PubMed).

Shibata, Hirano, Hedley-Whyte et al.: "Selective formation of certain advanced glycation end products in spinal cord astrocytes of humans and mice with superoxide dismutase-1 mutation." in: Acta neuropathologica, Vol. 104, Issue 2, pp. 171-8, 2002 (PubMed).