Pyrraline Antikörper

Antigen: Pyrraline

Klonalität: Monoklonal (H12)

Wirt: Maus

Reaktivität: Alle Spezies

Applikation: Immunhistochemie (IHC), ELISA

Produktnummer: ABIN289940

Produktbeschreibung

Immunogen: Pyrraline-HSA

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: H12

Beschreibung: Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base andAmadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recent immunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteinsin several human tissues: human lens (nondiabetic and noncataractous), renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves ofdiabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrilsin patients with hemodialysis-related amyloidosis, senile plaques of patients with Alzheimer

Anwendungen

Applikationshinweise: Working dilution for immunohistochemistry: about 2ug/ml, for ELISA: 0.1-0.5ug/ml

Konzentration: 0.25 mg/ml

Reinigung: The splenic lymphocytes from BALB/c mouse, immunized with pyrraline-HSA were fused to myeloma P3U1 cells. The cell line (H12) with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein Gaffinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar