AGEs Antikörper (Biotin)

Antigen: AGEs

Klonalität: Monoklonal (6D12)

Wirt: Maus

Reaktivität: Alle Spezies

Konjugat: Biotin

Applikation: Immunhistochemie (IHC), ELISA

Produktnummer: ABIN289931

Produktbeschreibung

Immunogen: AGEs-BSA

Format: Affinity-purified

Isotyp: IgG1

Klon: 6D12

Beschreibung: Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recentimmunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteinsin several human tissues: human lens (nondiabetic and noncataractous), renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves ofdiabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrilsin patients with hemodialysis-related amyloidosis, senile plaques of patients with Alzheimer

Spezifität: The initial study (Ref. 1) revealed that 6D12 does not recognize early products (Schiff base and Amadoriproducts), but shows a positive reaction to AGEs-samples obtained either from proteins, lysine derivatives ormonoamino-carboxylic acids, indicating the immunospecificity to a common structure among AGEs-structures.The subsequent study (Ref. 10) revealed of 6D12 is an Nepsilon- carboxymethyllysine(CML)-protein adduct.

Anwendungen

Applikationshinweise: Working dilution for immunohistochemistry: 2ug /mL, for ELISA: 0.1-0.5ug /mL, for WB : 0.25-5ug /mL

Konzentration: 0.25 mg/ml

Reinigung: The splenic lymphocytes from BALB/c mouse, immunized with AGEs-BSA were fused to myeloma P3U1 cells. The hybrid cells were screened, and the cell line(6D12) with positive reaction to AGEs-human serum albumin but negative to BSAwas selected through successive subclonings and grown in ascitic fluid of BALB/cmouse, from which the anti-AGEs antibody was purified by Protein G affinity chromatography (Reference No.1) and conjugated.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Lagerung: Store below -20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical staining of renal proximal tubules andglomeruli in patients with diabetic nephropathy, using anti-AGEs antibody 6D12 (Yamada, K. et al,.Clinical nephrology, Vol.42, 354-361, 1994)