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GFP Antikörper

Name: GFP Antikörper
Brand: antibodies-online
SKU: ABIN289429
Price: 2536.88 EUR
Availability: InStock

GFP Reaktivität: Aequorea victoria WB, ELISA, IHC Wirt: Ziege Polyclonal unconjugated

Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification).

1 image

(2 Referenzen)

Produktnummer ABIN289429

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Reaktivität
178

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Wirt
74

73

35

11

9

6

5

1
Ziege
Passende Sekundärantikörper
Klonalität
121

88
Polyklonal
Konjugat
105

14

12

9

6

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
173

87

56

42

33

32

31

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität (Details)

Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml

Aufreinigung

Affinity chromatography purified

Sterilität

Filtered

Immunogen

GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

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GFP Reaktivität: Aequorea victoria FACS, IM Wirt: Ziege Polyclonal FITC

Applikationshinweise

ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
Buser, Drubin: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).

Buser, McDonald: "Correlative GFP-immunoelectron microscopy in yeast." in: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung

GFP (GFP Produkte)

Synonyme

green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse

Viral Protein

Hintergrund

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.