Ratte anti-Maus IgM Antikörper (Biotin)
| Antigen |
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| Klonalität |
Monoklonal (EB121-15F9) |
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Wirt
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Reaktivität
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Human (187), Maus (128), Ratte (Rattus) (41), Huhn (27), Kaninchen (14), Rind (Kuh) (7), Affe (7), Hund (6), Schwein (6), Vogel (5), Ziege (5), Katze (3), Meerschweinchen (3), Pferd (3), Schaf (3)
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Konjugat
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FITC (78), HRP (57), Biotin (54), PE (34), Alkaline Phosphatase (AP) (31), TRITC (18), APC (13), Texas Red (TR) (13), PE,Cy7 (4), PerCP,Cy5.5 (4), DyLight 350 (3), DyLight 405 (3), DyLight 488 (3), DyLight 550 (3), DyLight 594 (3), DyLight 633 (3), DyLight 650 (3), DyLight 680 (3), DyLight 800 (3), Cy5 (2), PE,Cy5 (2), RPE (2), Rhodamine (2), SPRD (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Poly-HRP (1), beta-Gal (1)
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Applikation
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Durchflusszytometrie (FACS)
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| Produktnummer |
ABIN286922 |
| Menge |
500 ug (Varianten) |
| Preis |
188,75 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Isotyp
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IgG2a, kappa chain
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Klon
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EB121-15F9
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Spezifität
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The eB121 -15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121 -15F9 can be used as a detection secondary for mouse IgM., Mouse
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Applikationshinweise
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The eB121 -15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eB121 -15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.
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Buffer
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Phosphate buffer pH 7.2, 150 mM NaCl, 0.1% NaN3
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Lagerung
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Store at 4 °C. DO NOT FREEZE.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "IgM", Typ "Sekundärantikörper" finden
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Wirte
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Ziege (234), Maus (93), Ratte (55), Kaninchen (52), Schaf (7), Esel (2)
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Reaktivitäten
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Human (187), Maus (128), Ratte (Rattus) (41), Huhn (27), Kaninchen (14), Rind (Kuh) (7), Affe (7), Hund (6), Schwein (6), Vogel (5), Ziege (5), Katze (3), Meerschweinchen (3), Pferd (3), Schaf (3)
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Konjugate
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FITC (78), HRP (57), Biotin (54), PE (34), Alkaline Phosphatase (AP) (31), TRITC (18), APC (13), Texas Red (TR) (13), PE,Cy7 (4), PerCP,Cy5.5 (4), DyLight 350 (3), DyLight 405 (3), DyLight 488 (3), DyLight 550 (3), DyLight 594 (3), DyLight 633 (3), DyLight 650 (3), DyLight 680 (3), DyLight 800 (3), Cy5 (2), PE,Cy5 (2), RPE (2), Rhodamine (2), SPRD (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Poly-HRP (1), beta-Gal (1)
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Epitope
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Fc specific (25), Fc-Region, Chain mu (3)
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Alternativen 
