Interleukin 7 Receptor (IL7R) Antikörper (Alexa Fluor 488)

Antigen: Interleukin 7 Receptor (IL7R)

Synonyme: ILRA, CD127, IL7RA, CDW127, IL-7R-alpha, IL7R

Klonalität: Monoklonal (A7R34)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Alexa Fluor 488

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN286590

Produktbeschreibung

Weitere Bezeichnung: CD127

Isotyp: IgG2a, kappa chain

Klon: A7R34

Spezifität: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL -7 receptor. IL -7 receptor alpha chain is expressed by immature B cells in the bone marrow, double -negative (CD4 -/CD8 -), single -positive (CD4+ and CD8+), but not double -positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL -7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL -7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127., Mouse

Anwendungen

Applikationshinweise: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This A7R34 antibody has been reported for use in flow cytometric analysis.

Buffer: Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3, 0.1% gelatin

Lagerung: Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Staining of BALB/c splenocytes with APC anti -mouse CD3e (145 -2C11) (ABIN282622) and 0.5 µg of Alexa Fluor ® 488 Rat IgG2a Iso Cntrl (ABIN287234) (left) or 0.5 µg Alexa Fluor ® 488 anti -mouse CD127 (A7R34) (right). Cells in the lymphocyte gate were used for analysis.

anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) (Image 2)

Publikationen

Publikationen: Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).