3G11 Sialoganglioside Antigen Antikörper (Alexa Fluor 647)

Antigen: 3G11 Sialoganglioside Antigen

Klonalität: Monoklonal (SM3G11)

Wirt: Maus

Reaktivität: Maus, Ratte (Rattus)

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN286454

Produktbeschreibung

Isotyp: IgM, kappa chain

Klon: SM3G11

Spezifität: The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down -regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3epsilon, suggesting close proximity of these two proteins. Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11 - T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL -10., Mouse, Rat

Anwendungen

Applikationshinweise: This eBio3G11 (SM3G11, 3G11) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This eBio3G11 (SM3G11, 3G11) antibody has been reported for use in flow cytometric analysis.

Buffer: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization

Lagerung: Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Staining of C57Bl/6 splenocytes with FITC anti -mouse CD4 (GK1.5) (ABIN277606) and staining buffer (autofluorescence) (left) or 0.25 µg of Alexa Fluor ® 647 anti -mouse 3G11 (SM3G11) (right). Total viable cells were used for analysis.

anti-3G11 Sialoganglioside Antigen antibody (Alexa Fluor 647) (Image 2)