Smooth Muscle Actin Antikörper (N-Term)

Produktnummer ABIN2857027

Produktdetails anti-Smooth Muscle Actin Antikörper

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale: Rabbit polyclonal antibody to alpha Smooth Muscle Actin
alpha Smooth Muscle Actin antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Rat2 , C8D30 , NIH-3T3 , C2C12

Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.37 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Smooth Muscle Actin Antikörper (ABIN2857027)

Chang, Hsieh, Lu, Chen, Liang, Lin, Hung, Liou, Wu: "Therapeutic Targeting of Aristolochic Acid Induced Uremic Toxin Retention, SMAD 2/3 and JNK/ERK Pathways in Tubulointerstitial Fibrosis: Nephroprotective Role of Propolis in Chronic Kidney Disease." in: Toxins, Vol. 12, Issue 6, (2020) (PubMed).

Wang, Giusti, Ny, de Witte: "Nephrotoxic Effects in Zebrafish after Prolonged Exposure to Aristolochic Acid." in: Toxins, Vol. 12, Issue 4, (2020) (PubMed).

Sun, Lei, Huang et al.: "SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine ..." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 25, pp. 6244-6254, (2020) (PubMed).

Stone, Stainier: "Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium." in: Developmental cell, Vol. 50, Issue 2, pp. 247-255.e3, (2020) (PubMed).

Yeh, Chen, Liao, Wu, Huang, Lin: "The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia." in: International journal of molecular sciences, Vol. 21, Issue 5, (2020) (PubMed).

Cheong, Lai, Wu: "Connective tissue growth factor mediates transforming growth factor β-induced collagen expression in human endometrial stromal cells." in: PLoS ONE, Vol. 14, Issue 1, pp. e0210765, (2019) (PubMed).

Feng, Cheng, Yang, Zhang, Ji, Zhang, Lin, Li, Xiong, Liu, Jiang: "Guidance to rational use of pharmaceuticals in gallbladder sarcomatoid carcinoma using patient-derived cancer cells and whole exome sequencing." in: Oncotarget, Vol. 8, Issue 3, pp. 5349-5360, (2018) (PubMed).

Marro, Pfefferli, de Preux Charles, Bise, Jaźwińska: "Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration." in: PLoS ONE, Vol. 11, Issue 10, pp. e0165497, (2017) (PubMed).

McCracken, Catá, Crawford, Sinagoga, Schumacher, Rockich, Tsai, Mayhew, Spence, Zavros, Wells: "Modelling human development and disease in pluripotent stem-cell-derived gastric organoids." in: Nature, Vol. 516, Issue 7531, pp. 400-4, (2014) (PubMed).

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: actin alpha 2, smooth muscle (ACTA2 Produkte)

Hintergrund: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Cellular Localization: Cytoplasm , cytoskeleton

Molekulargewicht: 42 kDa

Gen-ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development