EGFR Antikörper

Produktnummer ABIN285476

Produktdetails anti-EGFR Antikörper

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunochromatography (IC)

Kreuzreaktivität: Maus, Ratte (Rattus), Pute

Aufreinigung: purified

Immunogen: EGFR antibody was raised in sheep using a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region as the immunogen.

Anwendungsinformationen

Applikationshinweise: WB: 10-50 µg/mL, IC: 25-100 µg/mL, IHC-P: 5 µg/mL, IP: 10-12 µg/mL, WB: 10-50 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: Protein G purified and supplied in PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Referenzen für anti-EGFR Antikörper (ABIN285476)

Saveri, De Luca, Nisi, Pisciotta, Romano, Piscosquito, Reilly, Polke, Cavallaro, Fabrizi, Fossa, Cichero, Lombardi, Lauria, Magri, Taroni, Pareyson, Bucci: "Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation." in: Cells, Vol. 9, Issue 4, (2020) (PubMed).

Larios, Mercier, Roux, Gruenberg: "ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes." in: The Journal of cell biology, Vol. 219, Issue 3, (2020) (PubMed).

Ripoll, Heiligenstein, Hurbain, Domingues, Figon, Petersen, Dennis, Houdusse, Marks, Raposo, Delevoye: "Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers." in: The Journal of cell biology, Vol. 217, Issue 8, pp. 2709-2726, (2019) (PubMed).

Matsui, Jiang, Nakano, Sakamaki, Yamamoto, Mizushima: "Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17." in: The Journal of cell biology, Vol. 217, Issue 8, pp. 2633-2645, (2019) (PubMed).

Keir, Chandramohan, Hemphill, Grandal, Melander, Pedersen, Horak, Kragh, Desjardins, Friedman, Bigner: "Sym004-induced EGFR elimination is associated with profound anti-tumor activity in EGFRvIII patient-derived glioblastoma models." in: Journal of neuro-oncology, Vol. 138, Issue 3, pp. 489-498, (2019) (PubMed).

Wenzel, Schultz, Schink, Pedersen, Nähse, Carlson, Brech, Stenmark, Raiborg: "Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation." in: Nature communications, Vol. 9, Issue 1, pp. 2932, (2018) (PubMed).

Torgersen, Klokk, Kavaliauskiene, Klose, Simons, Skotland, Sandvig: "The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport." in: Oncotarget, Vol. 7, Issue 52, pp. 86871-86888, (2018) (PubMed).

Filipek, de Araujo, Vogel, De Smet, Eberharter, Rebsamen, Rudashevskaya, Kremser, Yordanov, Tschaikner, Fürnrohr, Lechner, Dunzendorfer-Matt, Scheffzek, Bennett, Superti-Furga, Lindner, Stasyk, Huber: "LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning." in: The Journal of cell biology, Vol. 216, Issue 12, pp. 4199-4215, (2017) (PubMed).

Fujita, Huang, Lin, Groulx, Jean, Nguyen, Kuchitsu, Koyama-Honda, Mizushima, Fukuda, Kiger: "Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy." in: eLife, Vol. 6, (2017) (PubMed).

Lo Cicero, Delevoye, Gilles-Marsens, Loew, Dingli, Guéré, André, Vié, van Niel, Raposo: "Exosomes released by keratinocytes modulate melanocyte pigmentation." in: Nature communications, Vol. 6, pp. 7506, (2016) (PubMed).

Matsui, Noguchi, Fukuda: "Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts." in: The Journal of biological chemistry, Vol. 289, Issue 20, pp. 13986-95, (2014) (PubMed).

Rønning, Pedersen, Madshus, Stang: "CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor." in: Experimental cell research, Vol. 317, Issue 13, pp. 1804-16, (2011) (PubMed).

Larsen, Stockhausen, Poulsen: "Cell adhesion and EGFR activation regulate EphA2 expression in cancer." in: Cellular signalling, Vol. 22, Issue 4, pp. 636-44, (2010) (PubMed).

Grøvdal, Johannessen, Rødland, Madshus, Stang: "Dysregulation of Ack1 inhibits down-regulation of the EGF receptor." in: Experimental cell research, Vol. 314, Issue 6, pp. 1292-300, (2008) (PubMed).

Bertelsen, Breen, Sandvig, Stang, Madshus: "The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosis." in: Experimental cell research, Vol. 313, Issue 8, pp. 1696-709, (2007) (PubMed).

Oksvold, Huitfeldt, Langdon: "Identification of 14-3-3zeta as an EGF receptor interacting protein." in: FEBS letters, Vol. 569, Issue 1-3, pp. 207-10, (2004) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR (EGFR Produkte)

Synonyme: C-erb antikoerper, CG10079 antikoerper, D-EGFR antikoerper, D-Egf antikoerper, DEGFR antikoerper, DER antikoerper, DER flb antikoerper, DER/EGFR antikoerper, DER/faint little ball antikoerper, DER/top antikoerper, DER/torpedo antikoerper, DER1 antikoerper, DEgfr antikoerper, Degfr antikoerper, Der antikoerper, DmHD-33 antikoerper, Dmel\\CG10079 antikoerper, EFG-R antikoerper, EGF-R antikoerper, EGFR antikoerper, EGFr antikoerper, EGfr antikoerper, EK2-6 antikoerper, Egf antikoerper, Egf-r antikoerper, EgfR antikoerper, El antikoerper, Elp antikoerper, Elp-1 antikoerper, Elp-B1 antikoerper, Elp-B1RB1 antikoerper, HD-33 antikoerper, TOP antikoerper, Torpedo/DER antikoerper, Torpedo/Egfr antikoerper, c-erbB antikoerper, d-egf-r antikoerper, dEGFR antikoerper, dEGFR1 antikoerper, dEgfr antikoerper, der antikoerper, egfr antikoerper, flb antikoerper, l(2)05351 antikoerper, l(2)09261 antikoerper, l(2)57DEFa antikoerper, l(2)57EFa antikoerper, l(2)57Ea antikoerper, mor1 antikoerper, top antikoerper, top/DER antikoerper, top/flb antikoerper, torpedo/Egfr antikoerper, torpedo/egfr antikoerper, EGFR12 antikoerper, EGFR15 antikoerper, egfr1 antikoerper, Erbb2 antikoerper, ERBB antikoerper, ERBB1 antikoerper, HER1 antikoerper, PIG61 antikoerper, mENA antikoerper, ErbB-1 antikoerper, Errp antikoerper, 9030024J15Rik antikoerper, AI552599 antikoerper, Erbb antikoerper, Errb1 antikoerper, Wa5 antikoerper, wa-2 antikoerper, wa2 antikoerper, epidermal growth factor receptor antikoerper, Epidermal growth factor receptor antikoerper, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antikoerper, EGFR antikoerper, Egfr antikoerper, egfra antikoerper, egfr1 antikoerper, LOC5564544 antikoerper

Hintergrund: The epidermal growth factor receptor (EGFR) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha).

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine