FasL (CD178) Antikörper

Antigen: FasL (CD178)

Klonalität: Monoklonal (MFL4)

Wirt: Hamster

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Funktionale Studien (Func)

Produktnummer: ABIN282447

Produktbeschreibung

Weitere Bezeichnung: FasL

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: MFL4

Spezifität: The MFL4 monoclonal antibody reacts with mouse and rat Fas Ligand (CD178), a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by activated T cells. The interaction of FasL with its receptor CD95 induces Fas -mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse and rat antigens to MMPs has not been reported. MFL4 has been reported in the literature to cross -react with the rat FasL antigen., Mouse

Anwendungen

Applikationshinweise: The MFL4 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The MFL4 antibody has been reported for use in flow cytometric analysis. MFL4 has also been reported in blocking of FasL mediated killing in functional assays.

Buffer: Phosphate buffer pH 7.2,150 mM NaCl, No NaN3

Lagerung: Store at 4 °C.Avoid repeated freeze/thaw cycles.KEEP CONTENTS STERILE.Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Virologie, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of mouse Fas Ligand transfected cells with anti-mouse/rat CD178 (MFL4) Purified followed by anti-armenian hamster IgG Biotin and SA-PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.