4-1BBL Antikörper

Antigen: 4-1BBL

Klonalität: Monoklonal (TKS-1)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN280983

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: TKS-1

Spezifität: The TKS -1 monoclonal antibody reacts with mouse 4 -1BB Ligand (4 -1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4 -1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4 -1BBL with 4 -1BB (CDw137) is reported to deliver a costimulatory signal important in the T -APC interaction., Mouse

Anwendungen

Applikationshinweise: The TKS -1 antibody has been tested by flow cytometric analysis of resting mouse splenocytes and 2PK3 mouse cells. This can be used at less than or equal to 1 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The TKS -1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of 4 -1BB ligand in functional studies. (Please use Functional Grade purified TKS -1, cat. 16 -5901, in functional assays.)

Buffer: Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3

Lagerung: Store at 4 °C.Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Staining of BALB/c splenocytes (left) and 2PK3 cells (right) with 0.5 µg Purified Rat IgG2a Iso Cntrl (ABIN287084) (open histogram) or 0.5 µg Purified anti -mouse 4 -1BB Ligand (TKS -1) (colored histogram) followed by FITC Anti -Rat IgG (ABIN286871). Total viable cells were used for analysis.

anti-4-1BBL antibody (Image 2)