N-Cadherin (CD325) Antikörper

Antigen: N-Cadherin (CD325)

Klonalität: Monoklonal (8C11)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)

Produktnummer: ABIN280781

Produktbeschreibung

Format: Affinity-purified

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: 8C11

Spezifität: The 8C11 monoclonal antibody reacts with human CD325, also known as N -Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left -right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells., Human

Anwendungen

Applikationshinweise: This 8C11 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This 8C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Buffer: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3

Lagerung: Store at 4 °C.Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Staining of HeLa cells with 0.25 µg of Purified Mouse IgG1, K Iso Cntrl (ABIN287090) (open histogram) or 0.25 µg of Purified anti -human CD325 (8C11) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti -Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (ABIN286885). Total viable cells were used for analysis.