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ARHGEF4 Antikörper (N-Term)

ARHGEF4 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2787993
  • Target Alle ARHGEF4 Antikörper anzeigen
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Bindungsspezifität
    • 8
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen
    Wirt
    • 25
    • 5
    • 1
    Kaninchen
    Klonalität
    • 27
    • 4
    Polyklonal
    Konjugat
    • 11
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ARHGEF4 Antikörper ist unkonjugiert
    Applikation
    • 23
    • 17
    • 4
    • 1
    Western Blotting (WB)
    Sequenz
    ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
    Homologie
    Cow: 79%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
    Produktmerkmale
    This is a rabbit polyclonal antibody against Arhgef4. It was validated on Western Blot.
    Aufreinigung
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Arhgef4
  • Applikationshinweise
    Optimal working dilutions should be determined experimentally by the investigator.
    Kommentare

    Antigen size: 484 AA

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100106 (Western Blotting)
    'Independent Validation' Siegel
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100106
    Datum
    23.12.2016
    Antigen
    ARHGEF4
    Chargennummer
    QC30159-41213
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    primary mouse microglia
    Negativkontrolle
    Bewertung
    Passed. ABIN2787993 specifically recognizes Arhgef4 in primary mouse microglia lysates.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2787993
    Sekundärantikörper
    anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg, 10µg and 30µg total protein for 5min at 95°C in 5µl, 10µl and 20µl Laemmli SDS sample buffer and subsequently separate them on a denaturing self-made 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk for 1h at RT.
    • Wash membrane 3x 5min with TBST.
    • Incubation with primary rabbit anti-ARHGEF4 antibody (antibodies-online, ABIN2787993, lot QC30159-41213) diluted 1:500 in TBST containing 2% BSA ON at 4°C.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using ECL Plus Western Blotting Detection Reagent (GE Healthcare, RPN2132, lot 4645641) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    Anmerkungen
    The ARHGEF4 antibody ABIN2787993 reveals multiple bands as is expected for murine Arhgef4.
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
    Andere Bezeichnung
    Arhgef4 (ARHGEF4 Produkte)
    Synonyme
    ARHGEF4 antikoerper, ASEF antikoerper, ASEF1 antikoerper, GEF4 antikoerper, STM6 antikoerper, 9330140K16Rik antikoerper, Asef antikoerper, ENSMUSG00000070955 antikoerper, Rho guanine nucleotide exchange factor 4 antikoerper, uncharacterized LOC100470646 antikoerper, rho guanine nucleotide exchange factor 4 antikoerper, Rho guanine nucleotide exchange factor (GEF) 4 antikoerper, ARHGEF4 antikoerper, arhg4 antikoerper, LOC100470646 antikoerper, LOC100544917 antikoerper, Arhgef4 antikoerper
    Hintergrund
    Arhgef4 acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion By similarity. It is required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Arhgef4 is involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
    Alias Symbols: 9330140K16Rik, Asef, ENSMUSG00000070955
    Protein Size: 484
    Molekulargewicht
    57 kDa
    Gen-ID
    226970
    NCBI Accession
    NM_183019, NP_898840
    UniProt
    Q7TNR9
    Pathways
    Neurotrophin Signalübertragung
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