FEN1 Antikörper (C-Term)

Produktnummer ABIN2782483

Produktdetails anti-FEN1 Antikörper

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Schaf, Rind (Kuh), Pferd, Meerschweinchen, Kaninchen, Hund, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against FEN1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FEN1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 380 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: FEN1 (FEN1 Produkte)

Synonyme: FEN-1 antikoerper, FEN1 antikoerper, AW538437 antikoerper, MF1 antikoerper, RAD2 antikoerper, cb879 antikoerper, fen-1 antikoerper, fen1 antikoerper, mf1 antikoerper, rad2 antikoerper, xFEN1a antikoerper, flap structure-specific endonuclease 1 antikoerper, flap endonuclease 1-like antikoerper, Flap endonuclease-1 antikoerper, 5'-3' exonuclease family protein antikoerper, flap structure specific endonuclease 1 antikoerper, flap structure-specific endonuclease 1 L homeolog antikoerper, nuclease, Rad2 family antikoerper, FEN1 antikoerper, LOC100808044 antikoerper, Bm1_49605 antikoerper, AT5G26680 antikoerper, Fen1 antikoerper, fen1 antikoerper, fen1.L antikoerper

Hintergrund: FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Alias Symbols: FEN-1, MF1, RAD2
Protein Interaction Partner: SUMO2, SUMO3, PCNA, UBC, SHFM1, ELAC2, TNPO3, PPP2R5D, HARS, LPP, PFDN1, WRN, AICDA, RAD1, FN1, PAXIP1, APP, VRK1, RAP1A, cdt1, TERF1, RAD21, GSK3B, ENDOG, DDX11, NCAPG, EP300, BLM, EEF1G, ARHGDIA, HNRNPA1, CDK2, CDK1, APEX1, RAD9A, HUS1, CCNA2,
Protein Size: 380

Molekulargewicht: 42 kDa

Gen-ID: 2237

NCBI Accession: NM_004111, NP_004102

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA