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RHOT1 Antikörper (N-Term)

RHOT1 Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio) WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2782021
  • Target Alle RHOT1 Antikörper anzeigen
    RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
    Bindungsspezifität
    • 11
    • 8
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 37
    • 16
    • 15
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Zebrafisch (Danio rerio)
    Wirt
    • 30
    • 7
    Kaninchen
    Klonalität
    • 33
    • 4
    Polyklonal
    Konjugat
    • 20
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RHOT1 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 18
    • 4
    • 3
    • 3
    Western Blotting (WB)
    Sequenz
    EMKPACIKAL TRIFKISDQD NDGTLNDAEL NFFQRICFNT PLAPQALEDV
    Homologie
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
    Produktmerkmale
    This is a rabbit polyclonal antibody against RHOT1. It was validated on Western Blot using a cell lysate as a positive control.
    Aufreinigung
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human RHOT1
    Top Product
    Discover our top product RHOT1 Primärantikörper
  • Applikationshinweise
    Optimal working dilutions should be determined experimentally by the investigator.
    Kommentare

    Antigen size: 659 AA

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100008 (Western Blotting)
    'Independent Validation' Siegel
    by
    Shaw Lab, Department of Biochemistry, University of Utah
    No.
    #100008
    Datum
    12.09.2015
    Antigen
    RHOT1
    Chargennummer
    Validierte Anwendung
    Western Blotting
    Positivkontrolle

    Purified recombinant Miro1

    Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro1

    Negativkontrolle

    Purified recombinant Miro2

    Isolated mitochondria from immortalized Miro1 WT MEFs

    Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro2

    Bewertung

    Passed. ABIN405704 specifically recognizes Miro1 without cross-reaction with Miro2.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN405704
    Sekundärantikörper
    Goat-anti-rabbit IgG HRP conjugated (Sigma, A0545-1M22)
    Full Protocol
    • Transfect immortalized Miro1-/- MEFs with Myc-Miro1-V1 or Myc-Miro2-V1 as described in PMID 25136135.
    • Isolate mitochondria from immortalized Miro1+/+, Miro1-/-, Miro1-/- + Myc-Miro1 and Miro1-/- + Myc-Miro2 MEFs.
    • Determine protein concentration and snap freeze mitochondria in liquid nitrogen.
    • Thaw 30 µg mitochondria per lane on ice.
    • Thaw 100 ng recombinant Miro1 and Miro2 per lane on ice.
    • Add 2x Laemmli buffer and 2% BME to samples and denature for 5 min at 95 °C.
    • Separte 30 µg/lane mitochondria and 100 ng/lane recombinant protein on a 12.5% SDS-PAGE for 3 h at 40mA/180V.
    • Semi-dry transfer to PVDF membrane for 2 h at 250 mA/15 V.
    • Block the membrane with TBST + 5% non-fat dry milk for 1 h at RT.
    • Incubate with primary
      • rabbit anti-RHOT1 antibody (antibodies-online, ABIN405704) diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
      • rabbit anti-Myc1 antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
      • rabbit anti-Cyt c antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
    • Wash membrane 3x for 5 min with TBST buffer.
    • Incubate with secondar goat anti-rabbit IgG HRP antibody (Sigma, A0545-1M2) diluted 1:2000 in TBST + 5% non-fat dry milk for 1 h at RT.
    • Wash membrane 3x for 10 min with TBST buffer.
    • Reveal protein bands on a Bio-Rad imaging system using homemade ECL prepared according to PMID 17141265.
    • <
    Anmerkungen
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
    Andere Bezeichnung
    RHOT1 (RHOT1 Produkte)
    Synonyme
    rhot1 antikoerper, zgc:55581 antikoerper, zgc:77063 antikoerper, wu:fd14e11 antikoerper, wu:fi14a05 antikoerper, arht2 antikoerper, miro-2 antikoerper, rasl antikoerper, ARHT1 antikoerper, MIRO-1 antikoerper, MIRO1 antikoerper, 2210403N23Rik antikoerper, AA415293 antikoerper, AF244542 antikoerper, AI834919 antikoerper, Arht1 antikoerper, C430039G08Rik antikoerper, Miro1 antikoerper, miro1 antikoerper, si:dkeyp-97g3.6 antikoerper, ras homolog family member T1a antikoerper, ras homolog family member T2 antikoerper, ras homolog family member T1 antikoerper, ras homolog family member T1 L homeolog antikoerper, rhot1a antikoerper, rhot2 antikoerper, RHOT1 antikoerper, rhot1 antikoerper, rhot1.L antikoerper, Rhot1 antikoerper, rhot1b antikoerper
    Hintergrund
    RHOT1 is mitochondrial GTPase involved in mitochondrial trafficking. It is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
    Alias Symbols: ARHT1, FLJ11040, FLJ12633, MIRO-1, MIRO1
    Protein Interaction Partner: PCSK9, ERLIN2, PARK2, ILK, UBC, CFTR, PINK1, UBD, IRAK1, AMFR,
    Protein Size: 659
    Molekulargewicht
    75 kDa
    Gen-ID
    55288
    NCBI Accession
    NM_001033566, NP_001028738
    UniProt
    Q8IXI2
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