E2F3 Antikörper (C-Term)

Produktnummer ABIN2780388

Produktdetails anti-E2F3 Antikörper

Target: E2F3

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: LLQQTEDQIP SNLEGPFVNL LPPLLQEDYL LSLGEEEGIS DLFDAYDLEK

Spezifität: The immunizing peptide used to raise this antibody is 100 % homologous to isoform 2 (334aa, 37 kDa) of human E2F3.

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against E2F3. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human E2F3

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 334 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-E2F3 Antikörper (ABIN2780388)

Giangreco, Vaishnav, Wagner, Finelli, Fleshner, Van der Kwast, Vieth, Nonn: "Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue." in: Cancer prevention research (Philadelphia, Pa.), Vol. 6, Issue 5, pp. 483-94, (2013) (PubMed).

Melcher, Hartmann, Zopf, Herterich, Wilke, Müller, Rosler, Kudlich, Al-Taie, Rosenwald, Katzenberger, Scholtka, Seibold, Rogoll, Scheppach, Scheurlen, Lührs: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, (2011) (PubMed).

Naga Prasad, Duan, Gupta, Surampudi, Volinia, Calin, Liu, Kotwal, Moravec, Starling, Perez, Sen, Wu, Plow, Croce, Karnik: "Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27487-99, (2009) (PubMed).

Details zu E2F3

Target: E2F3

Abstract: E2F3 Produkte

Synonyme: E2F-3 antikoerper, E2F3b antikoerper, E2f3a antikoerper, mKIAA0075 antikoerper, RGD1561600 antikoerper, E2F transcription factor 3 antikoerper, E2F transcription factor 3 S homeolog antikoerper, E2F3 antikoerper, e2f3.S antikoerper, E2f3 antikoerper

Hintergrund: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
Alias Symbols: E2F-3
Protein Interaction Partner: RB1, TRIM28, TFDP1, HDAC1, SRA1, RBL1, FZR1, ELAVL1, PCNA, MCM4, HELLS, CCNB1, BCL6, ATAD2, HCFC1, USF1, KMT2D, RYBP, TFDP2, YY1, TFE3, SP1, CCNA2, CDK3, RNF144A, FHL2, WNK1, MGA, TEAD3, SPIB, PKIB, MYBL2, CREBBP, EAPP, JMY, PPP1R13B, TP53BP2, TP53INP1, B
Protein Size: 465

Molekulargewicht: 49 kDa

Gen-ID: 1871

NCBI Accession: NM_001243076

UniProt: Q68DT0