Mediator Complex Subunit 6 (MED6) (Middle Region) Antikörper

Details zu Produkt Nr. ABIN2778336, Anbieter: Anmelden zum Anzeigen
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
  • RNA polymerase transcriptional regulation mediator-like protein
  • mediator complex subunit 6
  • hypothetical protein
  • MED6
  • Med6
  • med6
  • CAALFM_C306170CA
Epitop
Middle Region
15
10
10
9
9
9
9
7
5
2
2
2
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
74
15
13
6
5
5
4
4
3
2
1
1
1
1
1
Wirt
Kaninchen
61
14
Klonalität
Polyklonal
Konjugat
Unkonjugiert
4
4
4
4
4
4
Applikation
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
71
38
3
3
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MED6
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN979955.
Sequenz LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV
Homologie Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 93%, Rat: 93%
Produktmerkmale This is a rabbit polyclonal antibody against MED6. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Affinity Purified
Andere Bezeichnung MED6 (MED6 Antibody Abstract)
Hintergrund MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: NY-REN-28
Protein Interaction Partner: TARDBP, UBC, CDK19, CDK8, MED10, MED23, CCNC, MED19, MED26, FBXW7, MED11, MED28, MED29, MED4, MED12, MED7, MED21, MED1, SMARCA4, BCL6, SUZ12, CUL1, SKP1, CTDP1, MED25, SREBF1, MED15, VDR, OBFC1, MED18, ZC3H13, TADA2A, RARA, PARP1, SMAD3, SMAD2, SMAD1, MED
Protein Size: 246
Molekulargewicht 28 kDa
Gen-ID 10001
NCBI Accession NM_005466, NP_005457
UniProt O75586
Forschungsgebiet Chromatin and Nuclear Signaling, Transcription Factors
Pathways Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 246 AA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778336) WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Allgemeine Veröffentlichungen Pavri, Lewis, Kim, Dilworth, Erdjument-Bromage, Tempst, de Murcia, Evans, Chambon, Reinberg: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

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