SRF (N-Term) Antikörper

Details zu Produkt Nr. ABIN2774664, Anbieter: Anmelden zum Anzeigen
Antigen
  • AW049942
  • AW240594
  • MCM1
  • SRFX
  • XSRF
  • srf-A
  • RGD1559787
  • srf
  • srf1
  • serum response factor
  • serum response factor L homeolog
  • serum response factor a
  • Srf
  • SRF
  • srf.L
  • srfa
Epitop
N-Term
23
21
7
6
6
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Human, Maus, Schwein, Kaninchen, Ratte (Rattus), Zebrafisch
155
112
78
5
4
4
3
2
2
1
1
1
1
1
Wirt
Kaninchen
125
17
14
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
112
52
45
39
30
8
7
7
3
2
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SRF
Sequenz ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM
Kreuzreaktivität Rind (Kuh), Hund, Huhn, Human, Maus, Ratte (Rattus)
Homologie Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Produktmerkmale This is a rabbit polyclonal antibody against SRF. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Protein A purified
Andere Bezeichnung Serum response factor (SRF)
Hintergrund SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Alias Symbols: MCM1
Protein Interaction Partner: Camk2d, ZIC3, ELK1, SRFBP1, SRF, NFKB1, PRKDC, CIRBP, ALDH3A1, SUMO1, UBE2I, SHBG, TRIM63, KDM6A, MYOCD, MKL1, CNTN2, CSNK2A1, SMARCA4, PELP1, KAT5, HDAC4, EPC1, SMAD7, HDAC3, MKL2, ATF6, BARX2, MAPKAPK2, FHL3, GATA4, CAMK2A, HOPX, PYCARD, NKX2-3, MINPP1,
Protein Size: 508
Molekulargewicht 52 kDa
Gen-ID 6722
NCBI Accession NM_003131, NP_003122
UniProt P11831
Applikationshinweise WB Suggested Anti-SRF Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 508 AA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:1562500
Chromatin Immunoprecipitation (ChIP) image for anti-SRF (N-Term) antibody (ABIN2774664) Chromatin Immunoprecipitation (ChIP) Using SRF Antibody - N-terminal region (P100866_...
Allgemeine Veröffentlichungen Doi, Iso, Yamazaki, Akiyama, Kanai, Sato, Kawai-Kowase, Tanaka, Maeno, Okamoto, Arai, Kedes, Kurabayashi: "HERP1 inhibits myocardin-induced vascular smooth muscle cell differentiation by interfering with SRF binding to CArG box." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 11, pp. 2328-34, 2005 (PubMed).

Haben Sie etwas anderes gesucht?