RFC5 Antikörper (N-Term)

Produktnummer ABIN2773876

Produktdetails anti-RFC5 Antikörper

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Pferd, Rind (Kuh), Saccharomyces cerevisiae, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFC5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Sequenz: METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE

Homologie: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Yeast: 90%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against RFC5. It was validated on Western Blot and immunohistochemistry.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFC5

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 340 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu RFC5

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Andere Bezeichnung: RFC5 (RFC5 Produkte)

Synonyme: zgc:110313 antikoerper, 2610020K06Rik antikoerper, 2610209F07Rik antikoerper, 36.5kDa antikoerper, 36kDa antikoerper, Recc5 antikoerper, RFC36 antikoerper, replication factor C (activator 1) 5 antikoerper, replication factor C subunit 5 antikoerper, replication factor C subunit 5 L homeolog antikoerper, rfc5 antikoerper, Rfc5 antikoerper, RFC5 antikoerper, rfc5.L antikoerper

Hintergrund: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: MGC1155, RFC36
Protein Interaction Partner: NAB2, XIAP, TRIM38, HUWE1, RFC4, SUMO2, PCNA, UBC, RPA3, RPA2, RPA1, EED, FBXO6, MMS19, VCP, ECT2, MDC1, MTMR2, RFC3, RFC2, RFC1, CAND1, CUL3, NEDD8, HDGF, SMARCAD1, PIDD1, MYC, RPAP3, MEPCE, RUVBL2, LRIF1, COPS6, MED31, RBM48, UNC119, EEF1A1, RAD17, BRD4
Protein Size: 340

Molekulargewicht: 38 kDa

Gen-ID: 5985

NCBI Accession: NM_007370, NP_031396

UniProt: P40937

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA