CD28 Antikörper (FITC)

Produktnummer ABIN2749055

Produktdetails anti-CD28 Antikörper (FITC)

Target: CD28

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Kreuzreaktivität (Details): Rat

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Mouse A20J B lymphoma cells transfected with rat CD28

Klon: JJ319

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 2 μg/mL.

Kommentare:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD28 Antikörper (FITC) (ABIN2749055)

Guillonneau, Séveno, Dugast, Li, Renaudin, Haspot, Usal, Veziers, Anegon, Vanhove: "Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8164-71, (2007) (PubMed).

Kerstan, Armbruster, Leverkus, Hünig: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).

van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, (2004) (PubMed).

Lühder, Huang, Dennehy, Guntermann, Müller, Winkler, Kerkau, Ikemizu, Davis, Hanke, Hünig: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, (2003) (PubMed).

Laskowski, Pratschke, Wilhelm, Dong, Beato, Taal, Gasser, Hancock, Sayegh, Tilney: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, (2002) (PubMed).

Haspot, Villemain, Laflamme, Coulon, Olive, Tiollier, Soulillou, Vanhove: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, (2002) (PubMed).

Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, (2001) (PubMed).

Dengler, Szabo, Sido, Nottmeyer, Zimmerman, Vahl, Hünig, Meuer: "Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation." in: Transplantation, Vol. 67, Issue 3, pp. 392-8, (1999) (PubMed).

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28 (CD28 Produkte)

Hintergrund: CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,Tp44

Gen-ID: 25660

UniProt: P31042

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response