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Antikörper
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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antikörper
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antikörper
| Antigen |
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| Synonyme |
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B |
| Klonalität |
Monoklonal (DCS-72) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
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| Produktnummer |
ABIN272326 |
| Menge |
100 µg (Lot specific) |
| Preis |
300,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: p27Kip1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27
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Weitere Bezeichnung
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CDKN1B / KIP1
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Swiss-Prot
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P46527
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Immunogen
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His-tagged recombinant full length Mouse p27 protein recognizing a 27kDa protein.
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Kreuzreaktivität
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Hund, Human, Affe, Maus, Ratte (Rattus)
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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DCS-72
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Beschreibung
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P27 associates with cyclin and cyclin-dependent kinase complexes to inhibit their kinaseactivity and contribute to the control of cell proliferation. p27 is phosphorylated on manysites, including threonine 187, in vivo, with the predominant phosphorylation site beingserine 10. The extent of serine 10 phosphorylation by proline-directed kinase is markedlyincreased in cells in the G(0)-G(1) phase of the cell cycle compared to cells in the S or Mphase. p27 concentration is regulated predominantly by post-translational mechanisms. p27 is degraded by both the ubiquitin-proteasome pathway and ubiquitinindependentproteolysis. Regulation of ubiquitin-mediated proteolysis is often achieved throughubiquitination of the targeted phosphorylated protein, which renders it more susceptibleto degradation.
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Spezifität
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The p27 kip1 functions as a Negative regulator of G1 mouse expression and has beenproposed to function as a possible mediator of TGFb induced G1 arrest. Species: Human, Mouse, Rat, Monkey and Canine (Dog). Others not tested. Add. Information: Predicted Molecular Weight: 22. 1 kDa
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Applikationshinweise
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Western Blot: 5-10 µg/ml. Immunoprecipitation: coprecipitates CDK4. Immunohistochemistry (Paraffin). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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Lot specific
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Reinigung
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Purified
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Buffer
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PBS containing 0.08% Sodium Azide as preservative.
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Lagerung
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Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Forschungsgebiet
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Krebs, Zellzyklus
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", Typ "Antikörper" finden
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Wirte
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Kaninchen (61), Maus (19), Ziege (8), Huhn (2)
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Reaktivitäten
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Human (81), Maus (35), Ratte (Rattus) (31), Hund (4), Katze (1), Huhn (1), Rind (Kuh) (1), Affe (1)
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Applikationen
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Western Blot (WB) (75), Immunhistochemie (Paraffinschnitte) (IHC (p)) (37), Enzyme Immunoassay (EIA) (18), Immunpräzipitation (IP) (18), ELISA (10), Immunfluoreszenz (IF) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Immunhistochemie (IHC) (9), ELISA (Detektionsantikörper) (5), Dot Blot (Dot) (3)
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Epitope
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pThr187 (5), internal (2), pSer178 (2), pThr157 (2), pThr198 (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer10 (1), pT157 (1), pT187 (1)
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