DARPP32 Antikörper

Produktnummer ABIN271882

Produktdetails anti-DARPP32 Antikörper

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DARPP32 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of DARPP-32 protein. (region surrounding Pro69)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Andere Bezeichnung: DARPP32 (PPP1R1B Produkte)

Synonyme: darpp32 antikoerper, darpp-32 antikoerper, Darpp-32 antikoerper, Darpp32 antikoerper, DARPP-32 antikoerper, DARPP32 antikoerper, AU040756 antikoerper, protein phosphatase 1 regulatory inhibitor subunit 1B antikoerper, protein phosphatase 1, regulatory (inhibitor) subunit 1B antikoerper, protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog antikoerper, ppp1r1b antikoerper, PPP1R1B antikoerper, Ppp1r1b antikoerper, ppp1r1b.S antikoerper

Hintergrund: Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

Molekulargewicht: approx. 32 kDa

Gen-ID: 84152

NCBI Accession: NP_001229393

UniProt: Q9UD71