WNK1 Antikörper

Produktnummer ABIN271862

Produktdetails anti-WNK1 Antikörper

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of WNK1 protein. (region surrounding Tyr52)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Andere Bezeichnung: WNK1 (WNK1 Produkte)

Synonyme: HSAN2 antikoerper, HSN2 antikoerper, KDP antikoerper, PRKWNK1 antikoerper, PSK antikoerper, p65 antikoerper, WNK1 antikoerper, kdp antikoerper, psk antikoerper, hsn2 antikoerper, hsan2 antikoerper, pha2c antikoerper, prkwnk1 antikoerper, 6430573H23Rik antikoerper, EG406236 antikoerper, Hsn2 antikoerper, Prkwnk1 antikoerper, mKIAA0344 antikoerper, ATWNK1 antikoerper, T9J14.14 antikoerper, T9J14_14 antikoerper, ZIK4 antikoerper, with no lysine (K) kinase 1 antikoerper, WNK lysine deficient protein kinase 1 antikoerper, WNK lysine deficient protein kinase 1 L homeolog antikoerper, serine/threonine-protein kinase WNK1 antikoerper, with no lysine (K) kinase 1 antikoerper, WNK1 antikoerper, wnk1.L antikoerper, wnk1 antikoerper, LOC100056745 antikoerper, Wnk1 antikoerper

Hintergrund: The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250 - 300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95 % homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion.Synonyms: Erythrocyte 65 kDa protein, KDP, KIAA0344, Kinase deficient protein, PRKWNK1, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, Serine/threonine-protein kinase WNK1, p65

Molekulargewicht: approx. 230 kDa

Gen-ID: 65125

NCBI Accession: NP_001171914

UniProt: Q9H4A3