SRF Antikörper

Produktnummer ABIN271823

Der Kaninchen Polyklonal anti-SRF Antikörper wird verwendet zum Nachweis von SRF in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für SRF Antikörper (ABIN271823)

Target: SRF (Serum Response Factor (SRF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SRF Antikörper

Spezifität: This antibody detects endogenous levels of SRF protein. (region surrounding Gly93)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu SRF

Target: SRF (Serum Response Factor (SRF))

Hintergrund: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF.

Molekulargewicht: approx. 58 kDa

Gen-ID: 6722

NCBI Accession: NP_003122

UniProt: P11831