ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) Antikörper

Antigen: ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3)

Synonyme: APG3, APG3L, PC3-96, FLJ22125, MGC15201, APG3-LIKE, DKFZp564M1178, Apg3l, Atg3l, 2610016C12Rik, Pig1, PIG-1, atg3, zgc:64156, wu:fb15g12

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN271683

Produktbeschreibung

Weitere Bezeichnung: APG3 / ATG3

Gen-ID: 64422, 171415

Immunogen: A synthetic peptide corresponding to the N-terminal of human ATG3 protein conjugatedto Blue Carrier Protein has been used as the immunogen. The peptide is homologouswith the corresponding sequence derived from ATG3 protein in mouse, rat, S.cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).

Beschreibung: FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier proteinconjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-likeenzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-likeenzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PEconjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. Thecomplex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 toATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 namedisoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed,with a highest expression in heart, skeletal muscle, kidney, liver and placenta.SIMILARITY: Belongs to the ATG3 family. Alternate Names: biosensis. Related Diseases: Autophagy

Spezifität: IHC, WB and ELISA confirmed the specificity for ATG3. Species Reactivity: Human, rat. Other species not yet tested.

Anwendungen

Applikationshinweise: IHC, WB, immunofluorescence. A dilution of 1:50 to 1:3000 dilution is recommended forthese applications. The optimal dilution should be determined by the end user. Fixation:In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 andincubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observedin HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).