ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae) (ATG16L1) Antikörper

Antigen: ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae) (ATG16L1)

Synonyme: IBD10, WDR30, APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, Apg16l, Atg16l, AI035639, AI225866, 1500009K01Rik, Wdr30, atg16, atg16l

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN271682

Produktbeschreibung

Weitere Bezeichnung: APG16L / ATG16L1

Gen-ID: 55054, 363278

Immunogen: A synthetic peptide corresponding to the N-terminal of human ATG16-L1 proteinconjugated to Blue Carrier Protein has been used as the immunogen. The peptide ishomologous with the corresponding sequence derived from isoform alpha of ATG16-Lprotein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris(dog).

Beschreibung: FUNCTION: Plays an essential role in autophagy. SUBUNIT: Homooligomer. Interactswith ATG5. Part of either the minor and major complexes respectively composed of 4sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1(800 kDa). SUBCELLULAR LOCATION: Cytoplasm. Preautophagosomal structure,preautophagosomal structure membrane, peripheral membrane protein. Localized topreautophagosomal structure (PAS) where it is involved in the membrane targeting ofATG5. ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.SIMILARITY: Belongs to the WD repeat ATG16 family. SIMILARITY: Contains 7 WDrepeats. Alternate Names: biosensis. Related Diseases: Autophagy

Spezifität: IHC, WB and ELISA confirmed the specificity for ATG16 L1. Species Reactivity: Human, rat. Other species not yet tested.

Anwendungen

Applikationshinweise: IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommendedfor these applications. The optimal dilution should be determined by the end user. Eosinophils seem to be stained using Rabbit antibody to APG16 L1 (ATG16L1): wholeserum (R-158-100). The cells stained for APG16 L1 (ATG16L1) appear in red. The cellswere then counter stained with Hoechst Dye (blue colour). Here, the merged picture ispresented.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Zheng, Ji, Li et al.: "Cloning and analysis of human Apg16L." in: DNA sequence : the journal of DNA sequencing and mapping, Vol. 15, Issue 4, pp. 303-5, 2004 (PubMed).