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CSF2RA Antikörper (Center)

CSF2RA Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN7300570
  • Target Alle CSF2RA Antikörper anzeigen
    CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
    Bindungsspezifität
    • 14
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reaktivität
    Human, Maus, Ratte
    Wirt
    • 44
    • 11
    Kaninchen
    Klonalität
    • 45
    • 10
    Polyklonal
    Konjugat
    • 27
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CSF2RA Antikörper ist unkonjugiert
    Applikation
    • 36
    • 23
    • 9
    • 8
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Spezifität
    Recognizes endogenous levels of CD116 protein.
    Produktmerkmale
    Rabbit polyclonal antibody to CD116
    Aufreinigung
    The antibody was purified by immunogen affinity chromatography.
    Immunogen
    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD116.
    Top Product
    Discover our top product CSF2RA Primärantikörper
  • Applikationshinweise
    WB (1:500 - 1:1000)
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100104 (Western Blotting)
    'Independent Validation' Siegel
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100104
    Datum
    23.12.2016
    Antigen
    CSF2RA
    Chargennummer
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    primary mouse microglia
    Negativkontrolle
    siRNA knock-down of CSF2RA in primary mouse microglia
    Bewertung
    Passed. ABIN2705734 specifically recognizes Csf2ra in primary mouse microglia lysates.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2705734
    Sekundärantikörper
    anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Transfect cells with 0.05nmol mouse CSF2RA Silencer Select siRNA (ThermoFisher Scientific, ASO1094M, ASO10991 and ASO109DF, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
    • Incubation with primary rabbit anti-CSF2RA antibody (antibodies-online, ABIN2705734, lot CP1D10A) diluted 1:500 in TBST for 1h at RT.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
    • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    Anmerkungen
    The RND3 antibody ABIN2705734 reveals a protein of the expected molecular weight of mouse Csf2ra in lysates of primary mouse microglia. The protein band’s intensity decreases upon knockdown with two of the three utilized siRNAs.
  • Format
    Liquid
    Buffer
    Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
    Andere Bezeichnung
    CD116 (CSF2RA Produkte)
    Synonyme
    CD116 antikoerper, CDw116 antikoerper, CSF2R antikoerper, CSF2RAX antikoerper, CSF2RAY antikoerper, CSF2RX antikoerper, CSF2RY antikoerper, GM-CSF-R-alpha antikoerper, GMCSFR antikoerper, GMR antikoerper, SMDP4 antikoerper, Csfgmra antikoerper, GM-CSF-Ra antikoerper, GM-CSFR antikoerper, colony stimulating factor 2 receptor, alpha antikoerper, colony stimulating factor 2 receptor alpha subunit antikoerper, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) antikoerper, CSF2RA antikoerper, Csf2ra antikoerper
    Hintergrund
    CSF2R, CSF2RY, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116, CD116
    Gen-ID
    1438, 12982
    UniProt
    P15509, Q00941
    Pathways
    JAK-STAT Signalweg
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