Netrin Receptor DCC Antikörper

Antigen: Netrin Receptor DCC

Klonalität: Monoklonal (AF5)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN270003

Produktbeschreibung

Gen-ID: 1630

Immunogen: The extracellular domain of the human DCC protein.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: AF5

Beschreibung: Invasive, metastatic colon cancer arises from pre-existing adenomas in both familial andsporadic cases and is characterized by the presence of multiple chromosomalalterations. In the progression from intramucosal carcinoma to invasive carcinoma,allelic loss on the long arm of chromosome 18 is frequently observed. Recently, a geneon 18q21.3 termed DCC, for deleted in colorectal carcinoma, has been isolated andshown to be deleted or mutated in both sporadic and inherited colon carcinoma whereasnormal colonic tissue retained and expressed the gene. Furthermore, loss of DCCexpression appears to accompany progression of adenomas to metastatic carcinoma.The inactivation of DCC suggests that it is a tumor suppressor gene. A functional rolefor DCC as a tumor suppressor gene is indicated by experiments demonstrating thatintroduction of a normal chromosome 18 into the human colon tumor cell lines COKFuor SW480.7 lacking DCC suppresses tumorigenicity. Analysis of the amino acidsequence deduced from cDNA clones indicates that DCC is a transmembraneglycoprotein consisting of 1447 amino acids with an extracellular domain havingextensive similarity to neural cell adhesion molecules (NCAM), a single transmembranesegment, and a unique cytoplasmic domain. The high degree of similarity to NCAMssuggests that DCC is involved in cell-cell interactions essential to the differentiated stateof the colonic epithelium. DCC expression has been observed in all tissues examinedexcept liver with the highest level of expression in brain tissue. Alterations in DCCexpression have also been observed in pancreatic adenocarcinoma and esophogealcarcinoma. However, DCC mRNA is expressed at very low levels, requiring reversetranscription followed by PCR amplification for unambiguous detection. Alternate Names: anti-Deleted Colorectal Carcinoma antibody.

Spezifität: This antibody recognises a band at approximately 190 kDa. Species Reactivity: Cross-reacts with Human. Not yet tested in other species. Localization: Cell Membrane and Secreted.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunofluorescence 1-5 ug/ml,immunoprecipitation 1-2 ug/ml,Western Blot Immunoblotting:1-5 ug/ml

Konzentration: 0.1 mg/ml

Buffer: 0.2% Gelatin, 0.05M Sodium phosphate. Preservative: less than 0.1% sodium azide.

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar