ATPase, Na+/K+ Transporting, alpha 1 Polypeptide (ATP1A1) Antikörper

Antigen: ATPase, Na+/K+ Transporting, alpha 1 Polypeptide (ATP1A1)

Synonyme: MGC3285, MGC51750, Atpa-1, BC010319, MGC38419, Nkaa1b, atp1a1, atp1a1a.1, ATP1A1

Klonalität: Monoklonal (9-A5)

Wirt: Maus

Reaktivität: Hund, Huhn, Human, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Immunpräzipitation (IP)

Produktnummer: ABIN269675

Produktbeschreibung

Weitere Bezeichnung: ATP1A1

Gen-ID: 476, 24211

Immunogen: Purified rat kidney sodium / potassium ATPase.

Format: Ascites

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 9-A5

Beschreibung: The sodium/potassium ATPase is an integral membrane enzyme found in all cells ofhigher organisms and is responsible for the ATP-dependent transport of sodium andpotassium across the cell membrane. This membrane-bound enzyme is related to anumber of other ATPases including sarcoplasmic and endoplasmic reticulum calciumATPase (SERCA) and plasma membrane calcium ATPase (PMCA). Thesodium/potassium ATPase consists of a large, multipass, transmembrane catalyticsubunit, termed the alpha subunit, and an associated smaller glycoprotein, termed thebeta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1,alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded bytwo multigene families.The different isoforms of the sodium/potassium ATPase exhibit tissue-specific anddevelopmental patterns of expression. The alpha 1 and beta mRNAs are present in allcell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restrictedpattern of cell-specific expression. The alpha subunit has been found in kidney, brain,heart, and to a lesser extent liver, skeletal and smooth muscle. Alternate Names: anti-ATP1A1 antibody, anti-Na+/K+ ATPase 1 antibody, anti-Sodium pump 1 antibody,anti-ATPase Na+/K+ transporting alpha 1 polypeptide antibody, anti-Na+/K+ ATPasealpha-1 subunit antibody, anti-Na+, K+ ATPase alpha subunit antibody, anti-alpha 1sodium potassium ATPase antibody, anti-sodium potassium ATPase alpha A catalyticpolypeptide antibody, anti-sodium pump subunit alpha-1 antibody, anti-MGC3285antibody, anti-MGC51750 antibody.

Spezifität: Species Reactivity: Reacts with Human, Rat, Dog and Chicken.

Anwendungen

Applikationshinweise: Immunohistochemistry 1/1000

Buffer: PBS. Preservative: 0.05% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ting-Beall, Beall, Hastings et al.: "Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy." in: FEBS letters, Vol. 265, Issue 1-2, pp. 121-5, 1990 (PubMed).

Ariyasu, Deerinck, Levinson et al.: "Distribution of (Na+ + K+)ATPase and sodium channels in skeletal muscle and electroplax." in: Journal of neurocytology, Vol. 16, Issue 4, pp. 511-22, 1988 (PubMed).

Sweadner: "Isozymes of the Na+/K+-ATPase." in: Biochimica et biophysica acta, Vol. 988, Issue 2, pp. 185-220, 1989 (PubMed).

Leffert, Schenk, Hubert et al.: "Hepatic (Na+,K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies." in: Hepatology (Baltimore, Md.), Vol. 5, Issue 3, pp. 501-7, 1985 (PubMed).

Ariyasu, Nichol, Ellisman: "Localization of sodium/potassium adenosine triphosphatase in multiple cell types of the murine nervous system with antibodies raised against the enzyme from kidney." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 10, pp. 2581-96, 1985 (PubMed).

Hubert, Schenk, Skelly et al.: "Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping." in: Biochemistry, Vol. 25, Issue 14, pp. 4156-63, 1986 (PubMed).

Schenk, Hubert, Leffert: "Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver." in: The Journal of biological chemistry, Vol. 259, Issue 23, pp. 14941-51, 1985 (PubMed).

Schenk, Leffert: "Monoclonal antibodies to rat Na+,K+-ATPase block enzymatic activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 17, pp. 5281-5, 1983 (PubMed).

Simon, Leffert, Ellisman et al.: "Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression." in: The American journal of physiology, Vol. 269, Issue 1 Pt 1, pp. C69-84, 1995 (PubMed).

Hou, Matherly: "Oligomeric structure of the human reduced folate carrier: identification of homo-oligomers and dominant-negative effects on carrier expression and function." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 3285-93, 2009 (PubMed).