MADCAM1 Antikörper

Produktnummer ABIN2689829

Produktdetails anti-MADCAM1 Antikörper

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser MADCAM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Produktmerkmale: The MECA-89 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γ can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin α4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes. Immunohistochemical staining of mouse MadCAM-1: Frozen sections of normal mouse small intestine were reacted with the anti-MadCAM-1 antibody. Cells of the lamina propria and the mucosal lymphoid tissue expressing MadCAM-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse MAdCAM-1 - Purified - Clone MECA-89 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse mesenteric and peripheral lymph node cells

Klon: MECA

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-MADCAM1 Antikörper (ABIN2689829)

Berg, McEvoy, Berlin, Bargatze, Butcher: "L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Streeter, Berg, Rouse, Bargatze, Butcher: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." in: Nature, Vol. 331, Issue 6151, pp. 41-6, (1988) (PubMed).

Details zu MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Andere Bezeichnung: MAdCAM-1 (MADCAM1 Produkte)

Synonyme: MACAM1 antikoerper, AV211525 antikoerper, mucosal vascular addressin cell adhesion molecule 1 antikoerper, MADCAM1 antikoerper, Madcam1 antikoerper