IRF3 Antikörper (AA 56-427)

Produktnummer ABIN967285

Produktdetails anti-IRF3 Antikörper

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Bindungsspezifität: AA 56-427

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IRF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IRF-3 recombinant fusion protein

Klon: SL-12-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Clone SL-12.1 can be used for western blot analysis (0.5-2.0 µg/ml). Other reported applications include immunoprecipitation and immunofluorescence microscopy. The antibody will recognize both the nuclear and cytoplasmic forms of IRF-3 and can block IRF-3 DNA binding. Jurkat cells are recommended as a positive control (Cat, No. ABIN968537).

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml

Buffer: Aqueous buffered solution.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-IRF3 Antikörper (ABIN967285)

Hiscott, Pitha, Genin, Nguyen, Heylbroeck, Mamane, Algarte, Lin: "Triggering the interferon response: the role of IRF-3 transcription factor." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 19, Issue 1, pp. 1-13, (1999) (PubMed).

Mamane, Heylbroeck, Génin, Algarté, Servant, LePage, DeLuca, Kwon, Lin, Hiscott: "Interferon regulatory factors: the next generation." in: Gene, Vol. 237, Issue 1, pp. 1-14, (1999) (PubMed).

Ronco, Karpova, Vidal, Howley: "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity." in: Genes & development, Vol. 12, Issue 13, pp. 2061-72, (1998) (PubMed).

Wathelet, Lin, Parekh, Ronco, Howley, Maniatis: "Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo." in: Molecular cell, Vol. 1, Issue 4, pp. 507-18, (1998) (PubMed).

Au, Moore, Lowther, Juang, Pitha: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, (1996) (PubMed).

Darnell, Kerr, Stark: "Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins." in: Science (New York, N.Y.), Vol. 264, Issue 5164, pp. 1415-21, (1994) (PubMed).

Loh, Chang, Fodor, Flavell: "Dissection of the interferon gamma-MHC class II signal transduction pathway reveals that type I and type II interferon systems share common signalling component(s)." in: The EMBO journal, Vol. 11, Issue 4, pp. 1351-63, (1992) (PubMed).

Reich, Evans, Levy, Fahey, Knight, Darnell: "Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6394-8, (1987) (PubMed).

Details zu IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Andere Bezeichnung: IRF-3 (IRF3 Produkte)

Synonyme: irf3 antikoerper, IRF3 antikoerper, C920001K05Rik antikoerper, IRF-3 antikoerper, interferon regulatory factor 3 antikoerper, interferon regulatory factor 7 antikoerper, irf3 antikoerper, IRF3 antikoerper, irf-3 antikoerper, Irf3 antikoerper, IRF7 antikoerper

Hintergrund: Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNalpha and IFNbeta). These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors that possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF-3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA. The antibody recognizes human IRF-3. A purified recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427) was used as the immunogen.

Molekulargewicht: 50 kDa

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades