CXCR5 Antikörper

Produktnummer ABIN2689482

Produktdetails anti-CXCR5 Antikörper

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CXCR5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The monoclonal antibody RF8B2 reacts with the human CXC chemokine receptor, CXCR5. CXCR5 (aka BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells suggested that this receptor might function as a regulator of B cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on human peripheral lymphocytes by purified RF8B2. Human peripheral blood mononuclear cells were stained with 0.25 μg/test of purified RF8B2 using 3-step staining protocol outlined above and anti-human CD3-FITC (Cat. No. 555339, left panel) or anti-human CD19 FITC (Cat. No. 555412, right panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2b (Cat. No. 555846 and FITC-conjugated mouse IgG2a (Cat. No. 555573) or FITC-conjugated mouse IgG1 (Cat. No. 555748) as isotype controls. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed using a BD FACScan™ Flow Cytometer

BD Pharmingen™ Purifed Rat Anti-Human CXCR5 - Purified - Clone RF8B2 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CXCR5

Klon: RF8B2

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CXCR5 Antikörper (ABIN2689482)

Gunn, Ngo, Ansel, Ekland, Cyster, Williams: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).

Legler, Loetscher, Roos, Clark-Lewis, Baggiolini, Moser: "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5." in: The Journal of experimental medicine, Vol. 187, Issue 4, pp. 655-60, (1998) (PubMed).

Förster, Emrich, Kremmer, Lipp: "Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells." in: Blood, Vol. 84, Issue 3, pp. 830-40, (1994) (PubMed).

Kouba, Vanetti, Wang, Schäfer, Höllt: "Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue." in: FEBS letters, Vol. 321, Issue 2-3, pp. 173-8, (1993) (PubMed).

Dobner, Wolf, Emrich, Lipp: "Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma." in: European journal of immunology, Vol. 22, Issue 11, pp. 2795-9, (1992) (PubMed).

Details zu CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Andere Bezeichnung: CXCR5 (CXCR5 Produkte)

Synonyme: BLR1 antikoerper, CRL1 antikoerper, CD185 antikoerper, MDR15 antikoerper, Blr1 antikoerper, CXC-R5 antikoerper, CXCR-5 antikoerper, Gpcr6 antikoerper, NLR antikoerper, C-X-C motif chemokine receptor 5 antikoerper, chemokine (C-X-C motif) receptor 5 antikoerper, CXCR5 antikoerper, Cxcr5 antikoerper